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231224s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/acs.langmuir.7b01139
|2 doi
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| 028 |
5 |
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|a pubmed24n0909.xml
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|a DE-627
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|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wu, Pei-Jung
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Hydrogel Patches on Live Cells through Surface-Mediated Polymerization
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| 264 |
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1 |
|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 22.01.2019
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|a Date Revised 22.01.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Many naturally occurring cells possess an intrinsic ability to cross biological barriers that block conventional drug delivery, and these cells offer a possible mode of active transport across the blood-brain barrier or into the core of tumor masses. While many technologies for the formation of complete, nanoparticle-loaded coatings on cells exist, a complete coating on the cell surface would disrupt the interaction of cells with their environments. To address this issue, cell surface patches that partially cover cell surfaces might provide a superior approach for cell-mediated therapeutic delivery. The goal of this study is to establish a simplified approach to producing polymeric patches of arbitrary shapes on a live cell via surface-mediated photopolymerization. Cell surfaces were nonspecifically labeled with eosin, and polyethylene (glycol) diacrylate (PEGDA) coatings were directed to specific sites using 530 nm irradiation through a chrome-coated photomask. These coatings may entrap drug-loaded or imaging particles. The extent of nonspecific formation of PEGDA hydrogel coatings increased with irradiation time, light intensity, and initiating species; 40 mW/cm2 irradiation for 5 min delivered high-resolution patterns on the surface of A549 cells, and these cells remained viable for 48 h postpatterning with fluorescent nanoparticle-loaded coatings. This work first demonstrated the feasibility of photopatterning polymer patches directly on the surface of cells
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| 650 |
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4 |
|a Journal Article
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|a Research Support, N.I.H., Extramural
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Hydrogels
|2 NLM
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| 650 |
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|a Serum Albumin, Bovine
|2 NLM
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| 650 |
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|a 27432CM55Q
|2 NLM
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| 650 |
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|a Polyethylene Glycols
|2 NLM
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| 650 |
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7 |
|a 3WJQ0SDW1A
|2 NLM
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| 700 |
1 |
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|a Lilly, Jacob L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arreaza, Roberto
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Berron, Brad J
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 27 vom: 11. Juli, Seite 6778-6784
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:33
|g year:2017
|g number:27
|g day:11
|g month:07
|g pages:6778-6784
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| 856 |
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|u http://dx.doi.org/10.1021/acs.langmuir.7b01139
|3 Volltext
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