Preparation of peptide-MHC and T-cell receptor dextramers by biotinylated dextran doping
Peptide-major histocompatibility complex (pMHC) multimers enable the detection, characterization, and isolation of antigen-specific T-cell subsets at the single-cell level via flow cytometry and fluorescence microscopy. These labeling reagents exploit a multivalent scaffold to increase the avidity o...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 62(2017), 3 vom: 01. März, Seite 123-130
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| 1. Verfasser: |
Bethune, Michael T
(VerfasserIn) |
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| Weitere Verfasser: |
Comin-Anduix, Begoña,
Hwang Fu, Yu-Hsien,
Ribas, Antoni,
Baltimore, David |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
T-cell receptor (TCR)
biotinylated dextran
cancer immunotherapy
dextramer
immunology
ligand discovery
major histocompatibility complex (MHC)
mehr...
multimer
pMHC
peptide
peptide-specific MHC clustering
tetramer
tumor antigen discovery
Dextrans
Fluorescent Dyes
Histocompatibility Antigens
Peptides
Receptors, Antigen, T-Cell
Recombinant Fusion Proteins
Biotin
6SO6U10H04
Streptavidin
9013-20-1 |
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