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231224s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.2144/000114524
|2 doi
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Xiao, Xia
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections
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1 |
|c 2017
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 24.10.2017
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|a Date Revised 06.03.2018
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|a published: Electronic
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|a Citation Status MEDLINE
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|a Fluorescent immunolabeling and imaging in free-floating thick (50-60 μm) tissue sections is relatively simple in practice and enables design-based non-biased stereology, or 3-D reconstruction and analysis. This method is widely used for 3-D in situ quantitative biology in many areas of biological research. However, the labeling quality and efficiency of standard protocols for fluorescent immunolabeling of these tissue sections are not always satisfactory. Here, we systematically evaluate the effects of raising the conventional antibody incubation temperatures (4°C or 21°C) to mammalian body temperature (37°C) in these protocols. Our modification significantly enhances the quality (labeling sensitivity, specificity, and homogeneity) and efficiency (antibody concentration and antibody incubation duration) of fluorescent immunolabeling of free-floating thick tissue sections
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4 |
|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a fluorescent immunolabeling
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4 |
|a free-floating thick tissue section
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4 |
|a three-dimensional quantification
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4 |
|a whole-mount
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| 650 |
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7 |
|a Antibodies
|2 NLM
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| 650 |
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7 |
|a Fluorescent Dyes
|2 NLM
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| 700 |
1 |
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|a Feng, Ya-Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Du, Bin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Han-Ru
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, You-Quan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qi, Jian-Guo
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 62(2017), 3 vom: 01. März, Seite 115-122
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:62
|g year:2017
|g number:3
|g day:01
|g month:03
|g pages:115-122
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/000114524
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|d 62
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