Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections
Fluorescent immunolabeling and imaging in free-floating thick (50-60 μm) tissue sections is relatively simple in practice and enables design-based non-biased stereology, or 3-D reconstruction and analysis. This method is widely used for 3-D in situ quantitative biology in many areas of biological re...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 62(2017), 3 vom: 01. März, Seite 115-122
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| 1. Verfasser: |
Xiao, Xia
(VerfasserIn) |
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| Weitere Verfasser: |
Feng, Ya-Ping,
Du, Bin,
Sun, Han-Ru,
Ding, You-Quan,
Qi, Jian-Guo |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
fluorescent immunolabeling
free-floating thick tissue section
three-dimensional quantification
whole-mount
Antibodies
Fluorescent Dyes |
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