Analytic and quality control validation and assessment of field performance of a point-of-care chemistry analyzer for use in the White rhinoceros
© 2017 American Society for Veterinary Clinical Pathology.
| Veröffentlicht in: | Veterinary clinical pathology. - 1975. - 46(2017), 1 vom: 02. März, Seite 100-110 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | Veterinary clinical pathology |
| Schlagworte: | Journal Article Validation Study Ceratotherium simum VetTest dry chemistry total allowable error wildlife |
| Zusammenfassung: | © 2017 American Society for Veterinary Clinical Pathology. BACKGROUND: A chemistry point-of-care analyzer would be useful for evaluating injured wildlife, particularly White rhinoceros (Ceratotherium simum) that survive poaching attempts. The IDEXX VetTest could be suitable, but species-specific validation, development of a statistical quality control (QC) strategy, and evaluation under field conditions are necessary OBJECTIVES: The objectives were to (1) validate the VetTest for the White rhinoceros, (2) perform QC validation on the VetTest and generate a statistical QC strategy, and (3) apply this QC strategy to monitor performance under typical field conditions METHODS: Differences between White rhinoceros heparin plasma and serum, short-term imprecision, and reportable range using rhinoceros plasma and long-term imprecision using commercial quality control material (QCM) were assessed against prescribed total allowable error (TEa ) for up to 15 analytes. Quality control validation was performed using data from the long-term imprecision study and TEa . A QC strategy using QCM was developed and used to monitor performance under field conditions RESULTS: Imprecision was acceptable for all analytes except for ALP, ALT, and AST at low activities. The reportable range for AST and LDH differed from the manufacturer's specifications. Eleven analytes were suitable for statistical QC using the 13s rule, 3 using the 2s rule; ALP was not suitable. In the field, observed error was < TEa for all 15 analytes and the sigma metric was > 3.0 for 12 analytes CONCLUSIONS: The VetTest is suitable for use in the White rhinoceros. Statistical QC is possible for most analytes and useful for evaluation of field performance |
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| Beschreibung: | Date Completed 26.09.2017 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.12456 |