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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2016.10.028
|2 doi
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|a pubmed24n1223.xml
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|a (NLM)27842297
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|a (PII)S0981-9428(16)30418-1
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Lim, Sun-Hyung
|e verfasserin
|4 aut
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|a RNAi-mediated suppression of dihydroflavonol 4-reductase in tobacco allows fine-tuning of flower color and flux through the flavonoid biosynthetic pathway
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.04.2017
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Masson SAS. All rights reserved.
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|a To examine flux regulation in the flavonoid pathway of tobacco flowers, we suppressed two genes for dihydroflavonol 4-reductase (NtDFR 1 and 2) by RNA interference (Ri)-mediated post transcriptional gene silencing in pink-flowered tobacco. Two phenotypes were observed, pale pink (DFR-Ri_PP)- and white (DFR-Ri_W)-flowered lines. The relative mRNA levels of NtDFR genes in DFR-Ri_PP and DFR-Ri_W lines were reduced by 79%-95% relative to non-transformed (NT) plants. DFR-Ri_W lines had five-fold higher levels of small interference RNAs compared to DFR-Ri_PP lines. Expression of eight structural genes in the flavonoid pathway was significantly increased in DFR-Ri_W lines but not in DFR-Ri_PP lines based on quantitative RT-PCR. Anthocyanin contents correlated with flower color, with a reduction of 72%-97% in DFR-Ri_PP and DFR-Ri_W lines. Decreases in anthocyanin in flower were proportional with reductions of proanthocyanidin content in seeds. Two pale pink lines, DFR-Ri_PP 17 and 20, with anthocyanin decreases and the lowest level of DFR gene silencing, had higher (dihydro) flavonol production than a white flowered line, DFR-Ri_W 67. This finding suggests that suppression of DFR can increase the total levels of flavonoids due to (dihydro) flavonol biosynthesis. Our observations that higher suppression of DFR had a greater influence on the expression of flavonoid biosynthetic genes demonstrates the key role of DFR in the pathway and allows selection among DFR-Ri lines for plants with specific gene expression profiles to fine-tune flux through the pathway
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|a Journal Article
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|a Anthocyanin
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|a DFR
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|a FLS
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|a Metabolic flux
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|a Post transcriptional gene silencing
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|a Proanthocyanidin
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|a Tobacco
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|a Flavonoids
|2 NLM
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|a Plant Proteins
|2 NLM
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|a RNA, Messenger
|2 NLM
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|a RNA, Plant
|2 NLM
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|a Alcohol Oxidoreductases
|2 NLM
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| 650 |
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|a EC 1.1.-
|2 NLM
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|a dihydroflavanol 4-reductase
|2 NLM
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|a EC 1.1.1.-
|2 NLM
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|a You, Min-Kyung
|e verfasserin
|4 aut
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|a Kim, Da-Hye
|e verfasserin
|4 aut
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|a Kim, Jae Kwang
|e verfasserin
|4 aut
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|a Lee, Jong-Yeol
|e verfasserin
|4 aut
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|a Ha, Sun-Hwa
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 109(2016) vom: 14. Dez., Seite 482-490
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:109
|g year:2016
|g day:14
|g month:12
|g pages:482-490
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|u http://dx.doi.org/10.1016/j.plaphy.2016.10.028
|3 Volltext
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