Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins

While nonspecific adsorption is widely used for immobilizing proteins on solid surfaces, the random nature of protein adsorption may reduce the activity of immobilized proteins due to occlusion of the active site. We hypothesized that the orientation a protein assumes on a given surface can be contr...

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Publié dans:	Langmuir : the ACS journal of surfaces and colloids. - 1985. - 32(2016), 34 vom: 30. Aug., Seite 8660-7
Auteur principal:	Cruz-Teran, Carlos A (Auteur)
Autres auteurs:	Carlin, Kevin B, Efimenko, Kirill, Genzer, Jan, Rao, Balaji M
Format:	Article en ligne
Langue:	English
Publié:	2016
Accès à la collection:	Langmuir : the ACS journal of surfaces and colloids
Sujets:	Journal Article Research Support, U.S. Gov't, Non-P.H.S. Archaeal Proteins DNA-Binding Proteins IgY Immobilized Proteins Immunoglobulins Mutant Proteins Peptide Library Sso7d protein, Sulfolobus plus... Silicon Dioxide 7631-86-9