Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins

While nonspecific adsorption is widely used for immobilizing proteins on solid surfaces, the random nature of protein adsorption may reduce the activity of immobilized proteins due to occlusion of the active site. We hypothesized that the orientation a protein assumes on a given surface can be contr...

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Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1999. - 32(2016), 34 vom: 30. Aug., Seite 8660-7
1. Verfasser:	Cruz-Teran, Carlos A (VerfasserIn)
Weitere Verfasser:	Carlin, Kevin B, Efimenko, Kirill, Genzer, Jan, Rao, Balaji M
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, U.S. Gov't, Non-P.H.S. Archaeal Proteins DNA-Binding Proteins IgY Immobilized Proteins Immunoglobulins Mutant Proteins Peptide Library Sso7d protein, Sulfolobus mehr... Silicon Dioxide 7631-86-9