A phased strategy to differentiate human CD14+monocytes into classically and alternatively activated macrophages and dendritic cells

There are currently several in vitro strategies to differentiate human CD14(+) monocytes isolated from peripheral blood mononuclear cells (PBMCs) into the M1 or M2 macrophage cell types. Each cell type is then verified using flow cytometric analysis of cell-surface markers. Human CD14(+) monocytes h...

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Publié dans:	BioTechniques. - 1991. - 61(2016), 1 vom: 07., Seite 33-41
Auteur principal:	Zarif, Jelani C (Auteur)
Autres auteurs:	Hernandez, James R, Verdone, James E, Campbell, Scott P, Drake, Charles G, Pienta, Kenneth J
Format:	Article en ligne
Langue:	English
Publié:	2016
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't M1 macrophages M2 macrophages cytokine macrophage polarization monocyte differentiation monocyte-derived dendritic cells multi-nucleated giant cells plus... peripheral blood mononuclear cells phased strategy IL6 protein, human Interleukin-6 Lipopolysaccharide Receptors