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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2016.05.005
|2 doi
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|a pubmed25n0871.xml
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|a (NLM)27297988
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|a (PII)S0168-9452(16)30074-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Zhang, Zhe
|e verfasserin
|4 aut
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| 245 |
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|a OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.03.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
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|a Cuticular wax, a hydrophobic layer on the surface of all aerial plant organs, has essential roles in plant growth and survival under various environments. Here we report a wax-deficient rice mutant oshsd1 with reduced epicuticular wax crystals and thicker cuticle membrane. Quantification of the wax components and fatty acids showed elevated levels of very-long-chain fatty acids (VLCFAs) and accumulation of soluble fatty acids in the leaves of the oshsd1 mutant. We determined the causative gene OsHSD1, a member of the short-chain dehydrogenase reductase family, through map-based cloning. It was ubiquitously expressed and responded to cold stress and exogenous treatments with NaCl or brassinosteroid analogs. Transient expression of OsHSD1-tagged green fluorescent protein revealed that OsHSD1 localized to both oil bodies and endoplasmic reticulum (ER). Dehydrogenase activity assays demonstrated that OsHSD1 was an NAD(+)/NADP(+)-dependent sterol dehydrogenase. Furthermore, OsHSD1 mutation resulted in faster protein degradation, but had no effect on the dehydrogenase activity. Together, our data indicated that OsHSD1 plays a specialized role in cuticle formation and lipid homeostasis, probably by mediating sterol signaling. This work provides new insights into oil-body associated proteins involved in wax and lipid metabolism
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|a Journal Article
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|a Cuticular wax
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|a Hydroxysteroid dehydrogenase
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|a Lipid
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|a Oil body
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|a Oryza sativa
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|a Plant Proteins
|2 NLM
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|a Hydroxysteroid Dehydrogenases
|2 NLM
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|a EC 1.1.-
|2 NLM
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| 700 |
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|a Cheng, Zhi-Jun
|e verfasserin
|4 aut
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|a Gan, Lu
|e verfasserin
|4 aut
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|a Zhang, Huan
|e verfasserin
|4 aut
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|a Wu, Fu-Qing
|e verfasserin
|4 aut
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|a Lin, Qi-Bing
|e verfasserin
|4 aut
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|a Wang, Jiu-Lin
|e verfasserin
|4 aut
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|a Wang, Jie
|e verfasserin
|4 aut
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|a Guo, Xiu-Ping
|e verfasserin
|4 aut
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|a Zhang, Xin
|e verfasserin
|4 aut
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|a Zhao, Zhi-Chao
|e verfasserin
|4 aut
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|a Lei, Cai-Lin
|e verfasserin
|4 aut
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|a Zhu, Shan-Shan
|e verfasserin
|4 aut
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|a Wang, Chun-Ming
|e verfasserin
|4 aut
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| 700 |
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|a Wan, Jian-Min
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 249(2016) vom: 01. Aug., Seite 35-45
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
1 |
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|g volume:249
|g year:2016
|g day:01
|g month:08
|g pages:35-45
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|u http://dx.doi.org/10.1016/j.plantsci.2016.05.005
|3 Volltext
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|d 249
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|h 35-45
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