OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 249(2016) vom: 01. Aug., Seite 35-45
1. Verfasser:	Zhang, Zhe (VerfasserIn)
Weitere Verfasser:	Cheng, Zhi-Jun, Gan, Lu, Zhang, Huan, Wu, Fu-Qing, Lin, Qi-Bing, Wang, Jiu-Lin, Wang, Jie, Guo, Xiu-Ping, Zhang, Xin, Zhao, Zhi-Chao, Lei, Cai-Lin, Zhu, Shan-Shan, Wang, Chun-Ming, Wan, Jian-Min
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Cuticular wax Hydroxysteroid dehydrogenase Lipid Oil body Oryza sativa Plant Proteins Hydroxysteroid Dehydrogenases EC 1.1.-

Beschreibung
Zusammenfassung:	Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. Cuticular wax, a hydrophobic layer on the surface of all aerial plant organs, has essential roles in plant growth and survival under various environments. Here we report a wax-deficient rice mutant oshsd1 with reduced epicuticular wax crystals and thicker cuticle membrane. Quantification of the wax components and fatty acids showed elevated levels of very-long-chain fatty acids (VLCFAs) and accumulation of soluble fatty acids in the leaves of the oshsd1 mutant. We determined the causative gene OsHSD1, a member of the short-chain dehydrogenase reductase family, through map-based cloning. It was ubiquitously expressed and responded to cold stress and exogenous treatments with NaCl or brassinosteroid analogs. Transient expression of OsHSD1-tagged green fluorescent protein revealed that OsHSD1 localized to both oil bodies and endoplasmic reticulum (ER). Dehydrogenase activity assays demonstrated that OsHSD1 was an NAD(+)/NADP(+)-dependent sterol dehydrogenase. Furthermore, OsHSD1 mutation resulted in faster protein degradation, but had no effect on the dehydrogenase activity. Together, our data indicated that OsHSD1 plays a specialized role in cuticle formation and lipid homeostasis, probably by mediating sterol signaling. This work provides new insights into oil-body associated proteins involved in wax and lipid metabolism
Beschreibung:	Date Completed 13.03.2017 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2016.05.005