Rapid and accurate synthesis of TALE genes from synthetic oligonucleotides

Custom synthesis of transcription activator-like effector (TALE) genes has relied upon plasmid libraries of pre-fabricated TALE-repeat monomers or oligomers. Here we describe a novel synthesis method that directly incorporates annealed synthetic oligonucleotides into the TALE-repeat units. Our appro...

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Détails bibliographiques

Publié dans:	BioTechniques. - 1993. - 60(2016), 6 vom: 01., Seite 299-305
Auteur principal:	Wang, Fenghua (Auteur)
Autres auteurs:	Zhang, Hefei, Gao, Jingxia, Chen, Fengjiao, Chen, Sijie, Zhang, Cuizhen, Peng, Gang
Format:	Article en ligne
Langue:	English
Publié:	2016
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't TALE gene synthesis genome engineering oligonucleotide Oligonucleotides Transcription Activator-Like Effectors