Rapid and accurate synthesis of TALE genes from synthetic oligonucleotides
Custom synthesis of transcription activator-like effector (TALE) genes has relied upon plasmid libraries of pre-fabricated TALE-repeat monomers or oligomers. Here we describe a novel synthesis method that directly incorporates annealed synthetic oligonucleotides into the TALE-repeat units. Our appro...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 60(2016), 6 vom: 01., Seite 299-305
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| 1. Verfasser: |
Wang, Fenghua
(VerfasserIn) |
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| Weitere Verfasser: |
Zhang, Hefei,
Gao, Jingxia,
Chen, Fengjiao,
Chen, Sijie,
Zhang, Cuizhen,
Peng, Gang |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2016
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
TALE
gene synthesis
genome engineering
oligonucleotide
Oligonucleotides
Transcription Activator-Like Effectors |
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