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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2016.03.020
|2 doi
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|a eng
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|a Rybaczek, Dorota
|e verfasserin
|4 aut
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|a Hydroxyurea-induced replication stress causes poly(ADP-ribose) polymerase-2 accumulation and changes its intranuclear location in root meristems of Vicia faba
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 10.04.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier GmbH. All rights reserved.
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|a Replication stress induced by 24 and 48h exposure to 2.5mM hydroxyurea (HU) increased the activity of poly(ADP-ribose) polymerase-2 (PARP-2; EC 2.4.2.30) in root meristem cells of Vicia faba. An increase in the number of PARP-2 foci was accompanied by their delocalization from peripheral areas to the interior of the nucleus. Our results indicate that the increase in PARP-2 was connected with an increase in S139-phosphorylated H2AX histones. The findings suggest the possible role of PARP-2 in replication stress. We also confirm that the intranuclear location of PARP-2 depends on the duration of HU-induced replication stress, confirming the role of PARP-2 as an indicator of stress intensity. Finally, we conclude that the more intense the HU-mediated replication stress, the greater the probability of PARP-2 activation or H2AXS139 phosphorylation, but also the greater the chance of increasing the efficiency of repair processes and a return to normal cell cycle progression
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|a Journal Article
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|a H2AX histone
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|a Hydroxyurea
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|a Poly(ADP-ribose) polymerase-2 (PARP-2)
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|a Replication stress
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|a Vicia faba
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|a Histones
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Poly(ADP-ribose) Polymerases
|2 NLM
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| 650 |
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|a EC 2.4.2.30
|2 NLM
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| 650 |
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|a Hydroxyurea
|2 NLM
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| 650 |
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|a X6Q56QN5QC
|2 NLM
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 198(2016) vom: 01. Juli, Seite 89-102
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:198
|g year:2016
|g day:01
|g month:07
|g pages:89-102
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|u http://dx.doi.org/10.1016/j.jplph.2016.03.020
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|d 198
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