Hydroxyurea-induced replication stress causes poly(ADP-ribose) polymerase-2 accumulation and changes its intranuclear location in root meristems of Vicia faba
Copyright © 2016 Elsevier GmbH. All rights reserved.
| Publié dans: | Journal of plant physiology. - 1979. - 198(2016) vom: 01. Juli, Seite 89-102 |
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| Auteur principal: | |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2016
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| Accès à la collection: | Journal of plant physiology |
| Sujets: | Journal Article H2AX histone Hydroxyurea Poly(ADP-ribose) polymerase-2 (PARP-2) Replication stress Vicia faba Histones Plant Proteins Poly(ADP-ribose) Polymerases EC 2.4.2.30 |
| Résumé: | Copyright © 2016 Elsevier GmbH. All rights reserved. Replication stress induced by 24 and 48h exposure to 2.5mM hydroxyurea (HU) increased the activity of poly(ADP-ribose) polymerase-2 (PARP-2; EC 2.4.2.30) in root meristem cells of Vicia faba. An increase in the number of PARP-2 foci was accompanied by their delocalization from peripheral areas to the interior of the nucleus. Our results indicate that the increase in PARP-2 was connected with an increase in S139-phosphorylated H2AX histones. The findings suggest the possible role of PARP-2 in replication stress. We also confirm that the intranuclear location of PARP-2 depends on the duration of HU-induced replication stress, confirming the role of PARP-2 as an indicator of stress intensity. Finally, we conclude that the more intense the HU-mediated replication stress, the greater the probability of PARP-2 activation or H2AXS139 phosphorylation, but also the greater the chance of increasing the efficiency of repair processes and a return to normal cell cycle progression |
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| Description: | Date Completed 10.04.2017 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2016.03.020 |