|
|
|
|
LEADER |
01000naa a22002652 4500 |
001 |
NLM258749717 |
003 |
DE-627 |
005 |
20231224185705.0 |
007 |
cr uuu---uuuuu |
008 |
231224s2012 xx |||||o 00| ||eng c |
024 |
7 |
|
|a 10.1111/j.1529-8817.2012.01137.x
|2 doi
|
028 |
5 |
2 |
|a pubmed24n0862.xml
|
035 |
|
|
|a (DE-627)NLM258749717
|
035 |
|
|
|a (NLM)27011073
|
040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
041 |
|
|
|a eng
|
100 |
1 |
|
|a Sanderson, Matthew C
|e verfasserin
|4 aut
|
245 |
1 |
2 |
|a A NOVEL ASSAY OF ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY UTILIZING FLUORESCENT SUBSTRATE(1)
|
264 |
|
1 |
|c 2012
|
336 |
|
|
|a Text
|b txt
|2 rdacontent
|
337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
500 |
|
|
|a Date Completed 26.03.2016
|
500 |
|
|
|a Date Revised 25.03.2016
|
500 |
|
|
|a published: Print-Electronic
|
500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
520 |
|
|
|a © 2012 Phycological Society of America.
|
520 |
|
|
|a Common methods for assaying acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymatic activity rely upon radiolabeled substrates or product assay. We developed a novel assay that directly quantifies endogenous DGAT activity through the use of a fluorescently labeled substrate. We performed this assay with microsomal protein, 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl-1-hexadecanoyl-sn-glycero-3-diacylglycerol (NBD-DAG), and oleoyl-CoA substrates. DGAT activity was analyzed in three species of algae as well as rat liver. The protocol proved to be sensitive and reliable. This assay may be used to facilitate research in the areas of biodiesel, oilseed crops, and triacylglycerol-related human pathologies
|
650 |
|
4 |
|a Journal Article
|
650 |
|
4 |
|a DGAT
|
650 |
|
4 |
|a biofuel
|
650 |
|
4 |
|a hyperlipidemia
|
650 |
|
4 |
|a lipids
|
650 |
|
4 |
|a oil
|
650 |
|
4 |
|a triacylglycerol
|
650 |
|
4 |
|a triglyceride
|
700 |
1 |
|
|a Venable, Mark E
|e verfasserin
|4 aut
|
773 |
0 |
8 |
|i Enthalten in
|t Journal of phycology
|d 1966
|g 48(2012), 3 vom: 24. Juni, Seite 580-4
|w (DE-627)NLM098182994
|x 1529-8817
|7 nnns
|
773 |
1 |
8 |
|g volume:48
|g year:2012
|g number:3
|g day:24
|g month:06
|g pages:580-4
|
856 |
4 |
0 |
|u http://dx.doi.org/10.1111/j.1529-8817.2012.01137.x
|3 Volltext
|
912 |
|
|
|a GBV_USEFLAG_A
|
912 |
|
|
|a SYSFLAG_A
|
912 |
|
|
|a GBV_NLM
|
912 |
|
|
|a GBV_ILN_21
|
912 |
|
|
|a GBV_ILN_40
|
912 |
|
|
|a GBV_ILN_72
|
912 |
|
|
|a GBV_ILN_176
|
912 |
|
|
|a GBV_ILN_350
|
951 |
|
|
|a AR
|
952 |
|
|
|d 48
|j 2012
|e 3
|b 24
|c 06
|h 580-4
|