A NOVEL ASSAY OF ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY UTILIZING FLUORESCENT SUBSTRATE(1)
© 2012 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 48(2012), 3 vom: 24. Juni, Seite 580-4 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article DGAT biofuel hyperlipidemia lipids oil triacylglycerol triglyceride |
| Zusammenfassung: | © 2012 Phycological Society of America. Common methods for assaying acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymatic activity rely upon radiolabeled substrates or product assay. We developed a novel assay that directly quantifies endogenous DGAT activity through the use of a fluorescently labeled substrate. We performed this assay with microsomal protein, 2-(6-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl-1-hexadecanoyl-sn-glycero-3-diacylglycerol (NBD-DAG), and oleoyl-CoA substrates. DGAT activity was analyzed in three species of algae as well as rat liver. The protocol proved to be sensitive and reliable. This assay may be used to facilitate research in the areas of biodiesel, oilseed crops, and triacylglycerol-related human pathologies |
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| Beschreibung: | Date Completed 26.03.2016 Date Revised 25.03.2016 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/j.1529-8817.2012.01137.x |