Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae) testing the influence of a light shift and diel periodicity

Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae) : testing the influence of a light shift and diel periodicity

© 2013 Phycological Society of America.

Bibliographische Detailangaben
Veröffentlicht in:Journal of phycology. - 1966. - 49(2013), 4 vom: 19. Aug., Seite 709-18
1. Verfasser: McGinley, Michael P (VerfasserIn)
Weitere Verfasser: Suggett, David J, Warner, Mark E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Journal of phycology
Schlagworte:Journal Article Symbiodinium dinoflagellates minicircle photosynthetic gene expression photosystem real-time PCR
Beschreibung
Zusammenfassung:© 2013 Phycological Society of America.
Microalgae possess numerous cellular mechanisms specifically employed for acclimating the photosynthetic pathways to changes in the physical environment. Despite the importance of coral-dinoflagellate symbioses, little focus has been given as to how the symbiotic algae (Symbiodinium spp.) regulate the expression of their photosynthetic genes. This study used real-time PCR to investigate the transcript abundance of the plastid-encoded genes, psbA (encoding the D1 protein of photosystem II) and psaA (encoding the P700 protein in photosystem I), within the cultured Symbiodinium ITS-2 (internal transcribed spacer region) types A20 and A13. Transcript abundance was monitored during a low to high-light shift, as well as over a full diel light cycle. In addition, psaA was characterized in three isolates (A20, A13, and D4-5) and noted as another example of a dinoflagellate plastid gene encoded on a minicircle. In general, the overall incongruence of transcript patterns for both psbA and psaA between the Symbiodinium isolates and other models of transcriptionally controlled chloroplast gene expression (e.g., Pisum sativum [pea], Sinapis alba [mustard seedling], and Synechocystis sp. PCC 6803 [cyanobacteria]) suggests that Symbiodinium is reliant on posttranscriptional mechanisms for homeostatic regulation of its photosynthetic proteins
Beschreibung:Date Completed 25.03.2016
Date Revised 24.03.2016
published: Print-Electronic
GENBANK: EU449053, JX624222, JX624223, JX624224, JX624225
Citation Status PubMed-not-MEDLINE
ISSN:1529-8817
DOI:10.1111/jpy.12079