Transcript patterns of chloroplast-encoded genes in cultured Symbiodinium spp. (Dinophyceae) : testing the influence of a light shift and diel periodicity
© 2013 Phycological Society of America.
| Publié dans: | Journal of phycology. - 1966. - 49(2013), 4 vom: 19. Aug., Seite 709-18 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2013
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| Accès à la collection: | Journal of phycology |
| Sujets: | Journal Article Symbiodinium dinoflagellates minicircle photosynthetic gene expression photosystem real-time PCR |
| Résumé: | © 2013 Phycological Society of America. Microalgae possess numerous cellular mechanisms specifically employed for acclimating the photosynthetic pathways to changes in the physical environment. Despite the importance of coral-dinoflagellate symbioses, little focus has been given as to how the symbiotic algae (Symbiodinium spp.) regulate the expression of their photosynthetic genes. This study used real-time PCR to investigate the transcript abundance of the plastid-encoded genes, psbA (encoding the D1 protein of photosystem II) and psaA (encoding the P700 protein in photosystem I), within the cultured Symbiodinium ITS-2 (internal transcribed spacer region) types A20 and A13. Transcript abundance was monitored during a low to high-light shift, as well as over a full diel light cycle. In addition, psaA was characterized in three isolates (A20, A13, and D4-5) and noted as another example of a dinoflagellate plastid gene encoded on a minicircle. In general, the overall incongruence of transcript patterns for both psbA and psaA between the Symbiodinium isolates and other models of transcriptionally controlled chloroplast gene expression (e.g., Pisum sativum [pea], Sinapis alba [mustard seedling], and Synechocystis sp. PCC 6803 [cyanobacteria]) suggests that Symbiodinium is reliant on posttranscriptional mechanisms for homeostatic regulation of its photosynthetic proteins |
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| Description: | Date Completed 25.03.2016 Date Revised 24.03.2016 published: Print-Electronic GENBANK: EU449053, JX624222, JX624223, JX624224, JX624225 Citation Status PubMed-not-MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.12079 |