|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM25853172X |
| 003 |
DE-627 |
| 005 |
20250219205746.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2014 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/jpy.12193
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0861.xml
|
| 035 |
|
|
|a (DE-627)NLM25853172X
|
| 035 |
|
|
|a (NLM)26988452
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Scholz, Bettina
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Purification and culture characteristics of 36 benthic marine diatoms isolated from the Solthörn tidal flat (Southern North Sea)
|
| 264 |
|
1 |
|c 2014
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 26.07.2016
|
| 500 |
|
|
|a Date Revised 18.03.2016
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a © 2014 Phycological Society of America.
|
| 520 |
|
|
|a Marine benthic diatoms growing in biofilms on sediment surfaces generally occur associated with heterotrophic bacteria, whereas modern molecular techniques and analyses of species-specific physiology create a demand for axenic cultures. Numerous benthic diatoms were isolated from surface sediments during a monitoring of the Solthörn tidal flat (southern North Sea, Germany) from May 2008 to May 2009. Of these, around 50% could be purified from the accompanying heterotrophic bacteria using different antibiotics combined with physical separation methods (vortexing, ultrasound). Overall, seven different antibiotics were tested at different concentrations, and a best working protocol was developed. The axenic strains were stable on average for only around 15 months, indicating a symbiotic interaction between the benthic diatoms and the associated bacteria. While most short-term effects during the purification process were restricted to differences in growth rates among xenic and axenic diatom strains, long-term cultivation led to distinct changes in cell volumes and growth characteristics of the axenic strains
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a antibiotics
|
| 650 |
|
4 |
|a axenic cultures
|
| 650 |
|
4 |
|a benthic marine diatoms
|
| 650 |
|
4 |
|a exopolysaccharides
|
| 650 |
|
4 |
|a physical pretreatments
|
| 650 |
|
4 |
|a strain stability
|
| 650 |
|
4 |
|a xenic cultures
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of phycology
|d 1966
|g 50(2014), 4 vom: 18. Aug., Seite 685-97
|w (DE-627)NLM098182994
|x 1529-8817
|7 nnas
|
| 773 |
1 |
8 |
|g volume:50
|g year:2014
|g number:4
|g day:18
|g month:08
|g pages:685-97
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jpy.12193
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_21
|
| 912 |
|
|
|a GBV_ILN_40
|
| 912 |
|
|
|a GBV_ILN_72
|
| 912 |
|
|
|a GBV_ILN_176
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 50
|j 2014
|e 4
|b 18
|c 08
|h 685-97
|