Purification and culture characteristics of 36 benthic marine diatoms isolated from the Solthörn tidal flat (Southern North Sea)
© 2014 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 50(2014), 4 vom: 18. Aug., Seite 685-97 |
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| 1. Verfasser: | |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article antibiotics axenic cultures benthic marine diatoms exopolysaccharides physical pretreatments strain stability xenic cultures |
| Zusammenfassung: | © 2014 Phycological Society of America. Marine benthic diatoms growing in biofilms on sediment surfaces generally occur associated with heterotrophic bacteria, whereas modern molecular techniques and analyses of species-specific physiology create a demand for axenic cultures. Numerous benthic diatoms were isolated from surface sediments during a monitoring of the Solthörn tidal flat (southern North Sea, Germany) from May 2008 to May 2009. Of these, around 50% could be purified from the accompanying heterotrophic bacteria using different antibiotics combined with physical separation methods (vortexing, ultrasound). Overall, seven different antibiotics were tested at different concentrations, and a best working protocol was developed. The axenic strains were stable on average for only around 15 months, indicating a symbiotic interaction between the benthic diatoms and the associated bacteria. While most short-term effects during the purification process were restricted to differences in growth rates among xenic and axenic diatom strains, long-term cultivation led to distinct changes in cell volumes and growth characteristics of the axenic strains |
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| Beschreibung: | Date Completed 26.07.2016 Date Revised 18.03.2016 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.12193 |