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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b04669
|2 doi
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|a pubmed24n0861.xml
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|a (DE-627)NLM258383453
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|a (NLM)26972953
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Castagna, Rossella
|e verfasserin
|4 aut
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|a Reactive Microcontact Printing of DNA Probes on (DMA-NAS-MAPS) Copolymer-Coated Substrates for Efficient Hybridization Platforms
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|c 2016
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 06.02.2017
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|a Date Revised 06.02.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a High-performing hybridization platforms fabricated by reactive microcontact printing of DNA probes are presented. Multishaped PDMS molds are used to covalently bind oligonucleotides over a functional copolymer (DMA-NAS-MAPS) surface. Printed structures with minimum width of about 1.5 μm, spaced by 10 μm, are demonstrated, with edge corrugation lower than 300 nm. The quantification of the immobilized surface probes via fluorescence imaging gives a remarkable concentration of 3.3 × 10(3) oligonucleotides/μm(2), almost totally active when used as probes in DNA-DNA hybridization assays. Indeed, fluorescence and atomic force microscopy show a 95% efficiency in target binding and uniform DNA hybridization over printed areas
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbocyanines
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|a DNA Probes
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|a DNA, Single-Stranded
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|a Fluorescent Dyes
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|a Methacrylates
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|a N,N-dimethylacrylamide-N-acryloyloxysuccinimide-3-(trimethoxysilyl)propyl methacrylate
|2 NLM
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|a Oligodeoxyribonucleotides
|2 NLM
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|a Succinimides
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|a cyanine dye 3
|2 NLM
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|a Bertucci, Alessandro
|e verfasserin
|4 aut
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|a Prasetyanto, Eko Adi
|e verfasserin
|4 aut
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|a Monticelli, Marco
|e verfasserin
|4 aut
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|a Conca, Dario Valter
|e verfasserin
|4 aut
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|a Massetti, Matteo
|e verfasserin
|4 aut
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|a Sharma, Parikshit Pratim
|e verfasserin
|4 aut
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|a Damin, Francesco
|e verfasserin
|4 aut
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|a Chiari, Marcella
|e verfasserin
|4 aut
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|a De Cola, Luisa
|e verfasserin
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|a Bertacco, Riccardo
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 13 vom: 05. Apr., Seite 3308-13
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:32
|g year:2016
|g number:13
|g day:05
|g month:04
|g pages:3308-13
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|u http://dx.doi.org/10.1021/acs.langmuir.5b04669
|3 Volltext
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