Reactive Microcontact Printing of DNA Probes on (DMA-NAS-MAPS) Copolymer-Coated Substrates for Efficient Hybridization Platforms

High-performing hybridization platforms fabricated by reactive microcontact printing of DNA probes are presented. Multishaped PDMS molds are used to covalently bind oligonucleotides over a functional copolymer (DMA-NAS-MAPS) surface. Printed structures with minimum width of about 1.5 μm, spaced by 1...

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Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 32(2016), 13 vom: 05. Apr., Seite 3308-13
1. Verfasser: Castagna, Rossella (VerfasserIn)
Weitere Verfasser: Bertucci, Alessandro, Prasetyanto, Eko Adi, Monticelli, Marco, Conca, Dario Valter, Massetti, Matteo, Sharma, Parikshit Pratim, Damin, Francesco, Chiari, Marcella, De Cola, Luisa, Bertacco, Riccardo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Carbocyanines DNA Probes DNA, Single-Stranded Fluorescent Dyes Methacrylates N,N-dimethylacrylamide-N-acryloyloxysuccinimide-3-(trimethoxysilyl)propyl methacrylate Oligodeoxyribonucleotides Succinimides cyanine dye 3
Beschreibung
Zusammenfassung:High-performing hybridization platforms fabricated by reactive microcontact printing of DNA probes are presented. Multishaped PDMS molds are used to covalently bind oligonucleotides over a functional copolymer (DMA-NAS-MAPS) surface. Printed structures with minimum width of about 1.5 μm, spaced by 10 μm, are demonstrated, with edge corrugation lower than 300 nm. The quantification of the immobilized surface probes via fluorescence imaging gives a remarkable concentration of 3.3 × 10(3) oligonucleotides/μm(2), almost totally active when used as probes in DNA-DNA hybridization assays. Indeed, fluorescence and atomic force microscopy show a 95% efficiency in target binding and uniform DNA hybridization over printed areas
Beschreibung:Date Completed 06.02.2017
Date Revised 06.02.2017
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/acs.langmuir.5b04669