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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2016.02.025
|2 doi
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|a pubmed24n0860.xml
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|a (DE-627)NLM258064293
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|a (NLM)26938582
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|a (PII)S0981-9428(16)30051-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Aznar-Moreno, Jose A
|e verfasserin
|4 aut
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|a Characterization of a small acyl-CoA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 13.12.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Masson SAS. All rights reserved.
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|a Acyl-CoA-binding proteins (ACBPs) bind to acyl-CoA esters and promote their interaction with other proteins, lipids and cell structures. Small class I ACBPs have been identified in different plants, such as Arabidopsis thaliana (AtACBP6), Brassica napus (BnACBP) and Oryza sativa (OsACBP1, OsACBP2, OsACBP3), and they are capable of binding to different acyl-CoA esters and phospholipids. Here we characterize HaACBP6, a class I ACBP expressed in sunflower (Helianthus annuus) tissues, studying the specificity of its corresponding recombinant HaACBP6 protein towards various acyl-CoA esters and phospholipids in vitro, particularly using isothermal titration calorimetry and protein phospholipid binding assays. This protein binds with high affinity to de novo synthetized derivatives palmitoly-CoA, stearoyl-CoA and oleoyl-CoA (Kd 0.29, 0.14 and 0.15 μM respectively). On the contrary, it showed lower affinity towards linoleoyl-CoA (Kd 5.6 μM). Moreover, rHaACBP6 binds to different phosphatidylcholine species (dipalmitoyl-PC, dioleoyl-PC and dilinoleoyl-PC), yet it displays no affinity towards other phospholipids like lyso-PC, phosphatidic acid and lysophosphatidic acid derivatives. In the light of these results, the possible involvement of this protein in sunflower oil synthesis is considered
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acyl-CoA
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|a Acyl-CoA binding protein
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|a Phosphatidic acid
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|a Phosphatidylcholine
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|a Phospholipids
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|a Sunflower
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|a Acyl Coenzyme A
|2 NLM
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| 650 |
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|a Carrier Proteins
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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|a Venegas-Calerón, Mónica
|e verfasserin
|4 aut
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| 700 |
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|a Du, Zhi-Yan
|e verfasserin
|4 aut
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| 700 |
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|a Garcés, Rafael
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tanner, Julian A
|e verfasserin
|4 aut
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|a Chye, Mee-Len
|e verfasserin
|4 aut
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|a Martínez-Force, Enrique
|e verfasserin
|4 aut
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|a Salas, Joaquín J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 102(2016) vom: 05. Mai, Seite 141-50
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:102
|g year:2016
|g day:05
|g month:05
|g pages:141-50
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|u http://dx.doi.org/10.1016/j.plaphy.2016.02.025
|3 Volltext
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|d 102
|j 2016
|b 05
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|h 141-50
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