Characterization of a small acyl-CoA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities

Characterization of a small acyl-CoA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities

Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 102(2016) vom: 05. Mai, Seite 141-50
1. Verfasser: Aznar-Moreno, Jose A (VerfasserIn)
Weitere Verfasser: Venegas-Calerón, Mónica, Du, Zhi-Yan, Garcés, Rafael, Tanner, Julian A, Chye, Mee-Len, Martínez-Force, Enrique, Salas, Joaquín J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Acyl-CoA Acyl-CoA binding protein Phosphatidic acid Phosphatidylcholine Phospholipids Sunflower Acyl Coenzyme A Carrier Proteins Plant Proteins
Beschreibung
Zusammenfassung:Copyright © 2016 Elsevier Masson SAS. All rights reserved.
Acyl-CoA-binding proteins (ACBPs) bind to acyl-CoA esters and promote their interaction with other proteins, lipids and cell structures. Small class I ACBPs have been identified in different plants, such as Arabidopsis thaliana (AtACBP6), Brassica napus (BnACBP) and Oryza sativa (OsACBP1, OsACBP2, OsACBP3), and they are capable of binding to different acyl-CoA esters and phospholipids. Here we characterize HaACBP6, a class I ACBP expressed in sunflower (Helianthus annuus) tissues, studying the specificity of its corresponding recombinant HaACBP6 protein towards various acyl-CoA esters and phospholipids in vitro, particularly using isothermal titration calorimetry and protein phospholipid binding assays. This protein binds with high affinity to de novo synthetized derivatives palmitoly-CoA, stearoyl-CoA and oleoyl-CoA (Kd 0.29, 0.14 and 0.15 μM respectively). On the contrary, it showed lower affinity towards linoleoyl-CoA (Kd 5.6 μM). Moreover, rHaACBP6 binds to different phosphatidylcholine species (dipalmitoyl-PC, dioleoyl-PC and dilinoleoyl-PC), yet it displays no affinity towards other phospholipids like lyso-PC, phosphatidic acid and lysophosphatidic acid derivatives. In the light of these results, the possible involvement of this protein in sunflower oil synthesis is considered
Beschreibung:Date Completed 13.12.2016
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2016.02.025