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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1155/2016/5076740
|2 doi
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|a pubmed25n0858.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhou, Peng
|e verfasserin
|4 aut
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| 245 |
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|a TLR4 Signaling in MPP⁺-Induced Activation of BV-2 Cells
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.11.2016
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|a Date Revised 13.11.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a AIMS: This work was conducted to establish an in vitro Parkinson's disease (PD) model by exposing BV-2 cells to 1-methyl-4-phenylpyridinium (MPP(+)) and exploring the roles of TLR2/TLR4/TLR9 in inflammatory responses to MPP(+)
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|a METHODS/RESULTS: MTT assay showed that cell viability of BV-2 cells was 84.78 ± 0.86% and 81.18 ± 0.99% of the control after incubation with 0.1 mM MPP(+) for 12 hours and 24 hours, respectively. Viability was not significantly different from the control group. With immunofluorescence technique, we found that MPP(+) incubation at 0.1 mM for 12 hours was the best condition to activate BV-2 cells. In this condition, the levels of TNF-α, IL-1β, and iNOS protein were statistically increased compared to the control according to ELISA tests. Real time RT-PCR and western blot measurements showed that TLR4 was statistically increased after 0.1 mM MPP(+) incubation for 12 hours. Furthermore, after siRNA interference of TLR4 mRNA, NF-κB activation and the levels of TNF-α, IL-1β, and iNOS were all statistically decreased in this cell model
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|a CONCLUSION: MPP(+) incubation at the concentration of 0.1 mM for 12 hours is the best condition to activate BV-2 cells for mimicking PD inflammation in BV-2 cells. TLR4 signalling plays a critical role in the activation of BV-2 cells and the induction of inflammation in this cell model
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Interleukin-1beta
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|a NF-kappa B
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|a RNA, Messenger
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|a Tlr4 protein, mouse
|2 NLM
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|a Toll-Like Receptor 4
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|a Tumor Necrosis Factor-alpha
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|a Nitric Oxide Synthase Type II
|2 NLM
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|a EC 1.14.13.39
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|a Nos2 protein, mouse
|2 NLM
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|a EC 1.14.13.39
|2 NLM
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|a 1-Methyl-4-phenylpyridinium
|2 NLM
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| 650 |
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|a R865A5OY8J
|2 NLM
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| 700 |
1 |
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|a Weng, Ruihui
|e verfasserin
|4 aut
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1 |
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|a Chen, Zhaoyu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Rui
|e verfasserin
|4 aut
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| 700 |
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|a Zou, Jing
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Xu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liao, Jinchi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yanping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xia, Ying
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Qing
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Neural plasticity
|d 1998
|g 2016(2016) vom: 15., Seite 5076740
|w (DE-627)NLM098558390
|x 1687-5443
|7 nnns
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| 773 |
1 |
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|g volume:2016
|g year:2016
|g day:15
|g pages:5076740
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|u http://dx.doi.org/10.1155/2016/5076740
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