TLR4 Signaling in MPP⁺-Induced Activation of BV-2 Cells

TLR4 Signaling in MPP⁺-Induced Activation of BV-2 Cells

AIMS: This work was conducted to establish an in vitro Parkinson's disease (PD) model by exposing BV-2 cells to 1-methyl-4-phenylpyridinium (MPP(+)) and exploring the roles of TLR2/TLR4/TLR9 in inflammatory responses to MPP(+)

Bibliographische Detailangaben
Veröffentlicht in:Neural plasticity. - 1998. - 2016(2016) vom: 15., Seite 5076740
1. Verfasser: Zhou, Peng (VerfasserIn)
Weitere Verfasser: Weng, Ruihui, Chen, Zhaoyu, Wang, Rui, Zou, Jing, Liu, Xu, Liao, Jinchi, Wang, Yanping, Xia, Ying, Wang, Qing
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Neural plasticity
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Interleukin-1beta NF-kappa B RNA, Messenger Tlr4 protein, mouse Toll-Like Receptor 4 Tumor Necrosis Factor-alpha Nitric Oxide Synthase Type II EC 1.14.13.39 mehr... Nos2 protein, mouse 1-Methyl-4-phenylpyridinium R865A5OY8J
Beschreibung
Zusammenfassung:AIMS: This work was conducted to establish an in vitro Parkinson's disease (PD) model by exposing BV-2 cells to 1-methyl-4-phenylpyridinium (MPP(+)) and exploring the roles of TLR2/TLR4/TLR9 in inflammatory responses to MPP(+)
METHODS/RESULTS: MTT assay showed that cell viability of BV-2 cells was 84.78 ± 0.86% and 81.18 ± 0.99% of the control after incubation with 0.1 mM MPP(+) for 12 hours and 24 hours, respectively. Viability was not significantly different from the control group. With immunofluorescence technique, we found that MPP(+) incubation at 0.1 mM for 12 hours was the best condition to activate BV-2 cells. In this condition, the levels of TNF-α, IL-1β, and iNOS protein were statistically increased compared to the control according to ELISA tests. Real time RT-PCR and western blot measurements showed that TLR4 was statistically increased after 0.1 mM MPP(+) incubation for 12 hours. Furthermore, after siRNA interference of TLR4 mRNA, NF-κB activation and the levels of TNF-α, IL-1β, and iNOS were all statistically decreased in this cell model
CONCLUSION: MPP(+) incubation at the concentration of 0.1 mM for 12 hours is the best condition to activate BV-2 cells for mimicking PD inflammation in BV-2 cells. TLR4 signalling plays a critical role in the activation of BV-2 cells and the induction of inflammation in this cell model
Beschreibung:Date Completed 09.11.2016
Date Revised 13.11.2018
published: Print-Electronic
Citation Status MEDLINE
ISSN:1687-5443
DOI:10.1155/2016/5076740