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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2016.01.010
|2 doi
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|a pubmed24n1223.xml
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|a (DE-627)NLM257488030
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|a (NLM)26878367
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|a (PII)S0176-1617(16)00017-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Corpas, Francisco J
|e verfasserin
|4 aut
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|a Activation of NADPH-recycling systems in leaves and roots of Arabidopsis thaliana under arsenic-induced stress conditions is accelerated by knock-out of Nudix hydrolase 19 (AtNUDX19) gene
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.08.2017
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier GmbH. All rights reserved.
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|a NADPH is an important cofactor in cell growth, proliferation and detoxification. Arabidopsis thaliana Nudix hydrolase 19 (AtNUDX19) belongs to a family of proteins defined by the conserved amino-acid sequence GX5-EX7REUXEEXGU which has the capacity to hydrolyze NADPH as a physiological substrate in vivo. Given the importance of NADPH in the cellular redox homeostasis of plants, the present study compares the responses of the main NADPH-recycling systems including NADP-isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH) and NADP-malic enzyme (ME) in the leaves and roots of Arabidopsis wild-type (Wt) and knock-out (KO) AtNUDX19 mutant (Atnudx19) plants under physiological and arsenic-induced stress conditions. Two major features were observed in the behavior of the main NADPH-recycling systems: (i) under optimal conditions in both organs, the levels of these activities were higher in nudx19 mutants than in Wt plants; and, (ii) under 500μM AsV conditions, these activities increase, especially in nudx19 mutant plants. Moreover, G6PDH activity in roots was the most affected enzyme in both Wt and nudx19 mutant plants, with a 4.6-fold and 5.0-fold increase, respectively. In summary, the data reveals a connection between the absence of chloroplastic AtNUDX19 and the rise in all NADP-dehydrogenase activities under physiological and arsenic-induced stress conditions, particularly in roots. This suggests that AtNUDX19 could be a key factor in modulating the NADPH pool in plants and consequently in redox homeostasis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 6PGDH
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|a Arsenic
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|a AtNUDX19
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|a G6PDH
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|a NADP-ICDH
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|a NADP-ME
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|a NADPH
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|a Oxidative stress
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|a Arabidopsis Proteins
|2 NLM
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|a NADP
|2 NLM
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|a 53-59-8
|2 NLM
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|a Malate Dehydrogenase
|2 NLM
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|a EC 1.1.1.37
|2 NLM
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|a malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
|2 NLM
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|a EC 1.1.1.40
|2 NLM
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|a Isocitrate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.41
|2 NLM
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| 650 |
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|a isocitrate dehydrogenase (NADP+)
|2 NLM
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| 650 |
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|a EC 1.1.1.42
|2 NLM
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|a Phosphogluconate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.43
|2 NLM
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| 650 |
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|a Glucosephosphate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.49
|2 NLM
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|a NADPH Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.6.99.1
|2 NLM
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|a Pyrophosphatases
|2 NLM
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|a EC 3.6.1.-
|2 NLM
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|a Arsenic
|2 NLM
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|a N712M78A8G
|2 NLM
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1 |
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|a Aguayo-Trinidad, Simeón
|e verfasserin
|4 aut
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|a Ogawa, Takahisa
|e verfasserin
|4 aut
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1 |
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|a Yoshimura, Kazuya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shigeoka, Shigeru
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 192(2016) vom: 15. März, Seite 81-9
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:192
|g year:2016
|g day:15
|g month:03
|g pages:81-9
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|u http://dx.doi.org/10.1016/j.jplph.2016.01.010
|3 Volltext
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|d 192
|j 2016
|b 15
|c 03
|h 81-9
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