Activation of NADPH-recycling systems in leaves and roots of Arabidopsis thaliana under arsenic-induced stress conditions is accelerated by knock-out of Nudix hydrolase 19 (AtNUDX19) gene

Bibliographische Detailangaben
Veröffentlicht in:	Journal of plant physiology. - 1979. - 192(2016) vom: 15. März, Seite 81-9
1. Verfasser:	Corpas, Francisco J (VerfasserIn)
Weitere Verfasser:	Aguayo-Trinidad, Simeón, Ogawa, Takahisa, Yoshimura, Kazuya, Shigeoka, Shigeru
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Journal of plant physiology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't 6PGDH Arsenic AtNUDX19 G6PDH NADP-ICDH NADP-ME NADPH Oxidative stress mehr... Arabidopsis Proteins NADP 53-59-8 Malate Dehydrogenase EC 1.1.1.37 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) EC 1.1.1.40 Isocitrate Dehydrogenase EC 1.1.1.41 isocitrate dehydrogenase (NADP+) EC 1.1.1.42 Phosphogluconate Dehydrogenase EC 1.1.1.43 Glucosephosphate Dehydrogenase EC 1.1.1.49 NADPH Dehydrogenase EC 1.6.99.1 Pyrophosphatases EC 3.6.1.- N712M78A8G

Beschreibung
Zusammenfassung:	Copyright © 2016 Elsevier GmbH. All rights reserved. NADPH is an important cofactor in cell growth, proliferation and detoxification. Arabidopsis thaliana Nudix hydrolase 19 (AtNUDX19) belongs to a family of proteins defined by the conserved amino-acid sequence GX5-EX7REUXEEXGU which has the capacity to hydrolyze NADPH as a physiological substrate in vivo. Given the importance of NADPH in the cellular redox homeostasis of plants, the present study compares the responses of the main NADPH-recycling systems including NADP-isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH) and NADP-malic enzyme (ME) in the leaves and roots of Arabidopsis wild-type (Wt) and knock-out (KO) AtNUDX19 mutant (Atnudx19) plants under physiological and arsenic-induced stress conditions. Two major features were observed in the behavior of the main NADPH-recycling systems: (i) under optimal conditions in both organs, the levels of these activities were higher in nudx19 mutants than in Wt plants; and, (ii) under 500μM AsV conditions, these activities increase, especially in nudx19 mutant plants. Moreover, G6PDH activity in roots was the most affected enzyme in both Wt and nudx19 mutant plants, with a 4.6-fold and 5.0-fold increase, respectively. In summary, the data reveals a connection between the absence of chloroplastic AtNUDX19 and the rise in all NADP-dehydrogenase activities under physiological and arsenic-induced stress conditions, particularly in roots. This suggests that AtNUDX19 could be a key factor in modulating the NADPH pool in plants and consequently in redox homeostasis
Beschreibung:	Date Completed 28.08.2017 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1618-1328
DOI:	10.1016/j.jplph.2016.01.010