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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2016.01.013
|2 doi
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|a pubmed24n0856.xml
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|a (DE-627)NLM257008543
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|a (NLM)26828405
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|a (PII)S0981-9428(16)30012-2
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Orawetz, Tom
|e verfasserin
|4 aut
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|a Reduction of the plastidial phosphorylase in potato (Solanum tuberosum L.) reveals impact on storage starch structure during growth at low temperature
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.11.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier Masson SAS. All rights reserved.
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|a Tubers of potato (Solanum tuberosum L.), one of the most important crops, are a prominent example for an efficient production of storage starch. Nevertheless, the synthesis of this storage starch is not completely understood. The plastidial phosphorylase (Pho1; EC 2.4.1.1) catalyzes the reversible transfer of glucosyl residues from glucose-1-phosphate to the non-reducing end of α-glucans with the release of orthophosphate. Thus, the enzyme is in principle able to act during starch synthesis. However, so far under normal growth conditions no alterations in tuber starch metabolism were observed. Based on analyses of other species and also from in vitro experiments with potato tuber slices it was supposed, that Pho1 has a stronger impact on starch metabolism, when plants grow under low temperature conditions. Therefore, we analyzed the starch content, granule size, as well as the internal structure of starch granules isolated from potato plants grown under low temperatures. Besides wild type, transgenic potato plants with a strong reduction in the Pho1 activity were analyzed. No significant alterations in starch content and granule size were detected. In contrast, when plants were cultivated at low temperatures the chain length distributions of the starch granules were altered. Thus, the granules contained more short glucan chains. That was not observed in the transgenic plants, revealing that Pho1 in wild type is involved in the formation of the short glucan chains, at least at low temperatures
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|a Journal Article
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|a Plastidial phosphorylase
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|a Potato
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|a Solanum tuberosum L.
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|a Starch granule
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|a Starch metabolism
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|a Starch synthase
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|a Plant Proteins
|2 NLM
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|a Starch
|2 NLM
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|a 9005-25-8
|2 NLM
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| 650 |
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|a Phosphorylases
|2 NLM
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| 650 |
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|a EC 2.4.1.-
|2 NLM
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| 700 |
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|a Malinova, Irina
|e verfasserin
|4 aut
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|a Orzechowski, Slawomir
|e verfasserin
|4 aut
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| 700 |
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|a Fettke, Joerg
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 100(2016) vom: 01. März, Seite 141-149
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:100
|g year:2016
|g day:01
|g month:03
|g pages:141-149
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|u http://dx.doi.org/10.1016/j.plaphy.2016.01.013
|3 Volltext
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