Membrane Protein Mobility and Orientation Preserved in Supported Bilayers Created Directly from Cell Plasma Membrane Blebs
Membrane protein interactions with lipids are crucial for their native biological behavior, yet traditional characterization methods are often carried out on purified protein in the absence of lipids. We present a simple method to transfer membrane proteins expressed in mammalian cells to an assay-f...
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Détails bibliographiques
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 32(2016), 12 vom: 29. März, Seite 2963-74
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| Auteur principal: |
Richards, Mark J
(Auteur) |
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| Autres auteurs: |
Hsia, Chih-Yun,
Singh, Rohit R,
Haider, Huma,
Kumpf, Julia,
Kawate, Toshimitsu,
Daniel, Susan |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2016
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids
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| Sujets: | Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
GPI-Linked Proteins
Lipid Bilayers
Liposomes
Luminescent Proteins
Phosphatidylcholines
Receptors, Purinergic P2X2
Green Fluorescent Proteins
147336-22-9
plus...
Formaldehyde
1HG84L3525
Dithiothreitol
T8ID5YZU6Y
1-palmitoyl-2-oleoylphosphatidylcholine
TE895536Y5 |
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