Membrane Protein Mobility and Orientation Preserved in Supported Bilayers Created Directly from Cell Plasma Membrane Blebs

Membrane protein interactions with lipids are crucial for their native biological behavior, yet traditional characterization methods are often carried out on purified protein in the absence of lipids. We present a simple method to transfer membrane proteins expressed in mammalian cells to an assay-f...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 32(2016), 12 vom: 29. März, Seite 2963-74
1. Verfasser: Richards, Mark J (VerfasserIn)
Weitere Verfasser: Hsia, Chih-Yun, Singh, Rohit R, Haider, Huma, Kumpf, Julia, Kawate, Toshimitsu, Daniel, Susan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. GPI-Linked Proteins Lipid Bilayers Liposomes Luminescent Proteins Phosphatidylcholines Receptors, Purinergic P2X2 Green Fluorescent Proteins 147336-22-9 mehr... Formaldehyde 1HG84L3525 Dithiothreitol T8ID5YZU6Y 1-palmitoyl-2-oleoylphosphatidylcholine TE895536Y5