Custom AFM for X-ray beamlines : in situ biological investigations under physiological conditions

A fast atomic force microscope (AFM) has been developed that can be installed as a sample holder for grazing-incidence X-ray experiments at solid/gas or solid/liquid interfaces. It allows a wide range of possible investigations, including soft and biological samples under physiological conditions (h...

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Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 22(2015), 6 vom: 11. Nov., Seite 1364-71
1. Verfasser: Gumí-Audenis, B (VerfasserIn)
Weitere Verfasser: Carlà, F, Vitorino, M V, Panzarella, A, Porcar, L, Boilot, M, Guerber, S, Bernard, P, Rodrigues, M S, Sanz, F, Giannotti, M I, Costa, L
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Evaluation Study Journal Article grazing-incidence scattering and reflectivity in situ atomic force microscopy model lipid membranes radiation damage Lipid Bilayers
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520 |a A fast atomic force microscope (AFM) has been developed that can be installed as a sample holder for grazing-incidence X-ray experiments at solid/gas or solid/liquid interfaces. It allows a wide range of possible investigations, including soft and biological samples under physiological conditions (hydrated specimens). The structural information obtained using the X-rays is combined with the data gathered with the AFM (morphology and mechanical properties), providing a unique characterization of the specimen and its dynamics in situ during an experiment. In this work, lipid monolayers and bilayers in air or liquid environment have been investigated by means of AFM, both with imaging and force spectroscopy, and X-ray reflectivity. In addition, this combination allows the radiation damage induced by the beam on the sample to be studied, as has been observed on DOPC and DPPC supported lipid bilayers under physiological conditions 
650 4 |a Evaluation Study 
650 4 |a Journal Article 
650 4 |a grazing-incidence scattering and reflectivity 
650 4 |a in situ atomic force microscopy 
650 4 |a model lipid membranes 
650 4 |a radiation damage 
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700 1 |a Carlà, F  |e verfasserin  |4 aut 
700 1 |a Vitorino, M V  |e verfasserin  |4 aut 
700 1 |a Panzarella, A  |e verfasserin  |4 aut 
700 1 |a Porcar, L  |e verfasserin  |4 aut 
700 1 |a Boilot, M  |e verfasserin  |4 aut 
700 1 |a Guerber, S  |e verfasserin  |4 aut 
700 1 |a Bernard, P  |e verfasserin  |4 aut 
700 1 |a Rodrigues, M S  |e verfasserin  |4 aut 
700 1 |a Sanz, F  |e verfasserin  |4 aut 
700 1 |a Giannotti, M I  |e verfasserin  |4 aut 
700 1 |a Costa, L  |e verfasserin  |4 aut 
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