Custom AFM for X-ray beamlines in situ biological investigations under physiological conditions

Custom AFM for X-ray beamlines : in situ biological investigations under physiological conditions

A fast atomic force microscope (AFM) has been developed that can be installed as a sample holder for grazing-incidence X-ray experiments at solid/gas or solid/liquid interfaces. It allows a wide range of possible investigations, including soft and biological samples under physiological conditions (h...

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Bibliographische Detailangaben
Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 22(2015), 6 vom: 11. Nov., Seite 1364-71
1. Verfasser: Gumí-Audenis, B (VerfasserIn)
Weitere Verfasser: Carlà, F, Vitorino, M V, Panzarella, A, Porcar, L, Boilot, M, Guerber, S, Bernard, P, Rodrigues, M S, Sanz, F, Giannotti, M I, Costa, L
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Evaluation Study Journal Article grazing-incidence scattering and reflectivity in situ atomic force microscopy model lipid membranes radiation damage Lipid Bilayers
Beschreibung
Zusammenfassung:A fast atomic force microscope (AFM) has been developed that can be installed as a sample holder for grazing-incidence X-ray experiments at solid/gas or solid/liquid interfaces. It allows a wide range of possible investigations, including soft and biological samples under physiological conditions (hydrated specimens). The structural information obtained using the X-rays is combined with the data gathered with the AFM (morphology and mechanical properties), providing a unique characterization of the specimen and its dynamics in situ during an experiment. In this work, lipid monolayers and bilayers in air or liquid environment have been investigated by means of AFM, both with imaging and force spectroscopy, and X-ray reflectivity. In addition, this combination allows the radiation damage induced by the beam on the sample to be studied, as has been observed on DOPC and DPPC supported lipid bilayers under physiological conditions
Beschreibung:Date Completed 15.08.2016
Date Revised 11.11.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1600-5775
DOI:10.1107/S1600577515016318