Identification and regulation of host genes related to Rice stripe virus symptom production

Identification and regulation of host genes related to Rice stripe virus symptom production

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 209(2016), 3 vom: 11. Feb., Seite 1106-19
1. Verfasser: Shi, Bingbin (VerfasserIn)
Weitere Verfasser: Lin, Lin, Wang, Shihui, Guo, Qin, Zhou, Hong, Rong, Lingling, Li, Junmin, Peng, Jiejun, Lu, Yuwen, Zheng, Hongying, Yang, Yong, Chen, Zhuo, Zhao, Jinping, Jiang, Tong, Song, Baoan, Chen, Jianping, Yan, Fei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Nicotiana benthamiana Oryza sativa Rice stripe virus (RSV) chloroplast-related genes chlorosis eIF4A symptom development virus-derived siRNA mehr... Plant Proteins RNA, Messenger RNA, Small Interfering
LEADER 01000caa a22002652 4500
001 NLM253881501
003 DE-627
005 20231227125250.0
007 cr uuu---uuuuu
008 231224s2016 xx |||||o 00| ||eng c
024 7 |a 10.1111/nph.13699  |2 doi 
028 5 2 |a pubmed24n1223.xml 
035 |a (DE-627)NLM253881501 
035 |a (NLM)26487490 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Shi, Bingbin  |e verfasserin  |4 aut 
245 1 0 |a Identification and regulation of host genes related to Rice stripe virus symptom production 
264 1 |c 2016 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 13.12.2016 
500 |a Date Revised 13.12.2023 
500 |a published: Print-Electronic 
500 |a GENBANK: AB026056.1, KP979608 
500 |a Citation Status MEDLINE 
520 |a © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. 
520 |a Viral infections cause plant chlorosis, stunting, necrosis or other symptoms. The down-regulation of chloroplast-related genes (ChRGs) is assumed to be responsible for chlorosis. We identified the differentially expressed genes (DEGs) in Rice stripe virus (RSV)-infected Nicotiana benthamiana, and examined the contribution of 75 down-regulated DEGs to RSV symptoms by silencing them one by one using Tobacco rattle virus (TRV)-induced gene silencing. Silencing of 11 of the 75 down-regulated DEGs caused plant chlorosis, and nine of the 11 were ChRGs. Silencing of a down-regulated DEG encoding the eukaryotic translation initiation factor 4A (eIF4A) caused leaf-twisting and stunting that were visible on RSV-infected N. benthamiana. A region of RSV RNA4 was complementary to part of eIF4A mRNA and virus-derived small interfering (vsiRNAs) from that region were present in infected N. benthamiana. When expressed as artificial microRNAs, those vsiRNAs could target NbeIF4A mRNA for regulation. We provide experimental evidence supporting the association of ChRGs with chlorosis and show that eIF4A is involved in RSV symptom development. This is also the first report demonstrating that siRNA derived directly from a plant virus can target a host gene for regulation 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Nicotiana benthamiana 
650 4 |a Oryza sativa 
650 4 |a Rice stripe virus (RSV) 
650 4 |a chloroplast-related genes 
650 4 |a chlorosis 
650 4 |a eIF4A 
650 4 |a symptom development 
650 4 |a virus-derived siRNA 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a RNA, Small Interfering  |2 NLM 
700 1 |a Lin, Lin  |e verfasserin  |4 aut 
700 1 |a Wang, Shihui  |e verfasserin  |4 aut 
700 1 |a Guo, Qin  |e verfasserin  |4 aut 
700 1 |a Zhou, Hong  |e verfasserin  |4 aut 
700 1 |a Rong, Lingling  |e verfasserin  |4 aut 
700 1 |a Li, Junmin  |e verfasserin  |4 aut 
700 1 |a Peng, Jiejun  |e verfasserin  |4 aut 
700 1 |a Lu, Yuwen  |e verfasserin  |4 aut 
700 1 |a Zheng, Hongying  |e verfasserin  |4 aut 
700 1 |a Yang, Yong  |e verfasserin  |4 aut 
700 1 |a Chen, Zhuo  |e verfasserin  |4 aut 
700 1 |a Zhao, Jinping  |e verfasserin  |4 aut 
700 1 |a Jiang, Tong  |e verfasserin  |4 aut 
700 1 |a Song, Baoan  |e verfasserin  |4 aut 
700 1 |a Chen, Jianping  |e verfasserin  |4 aut 
700 1 |a Yan, Fei  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 209(2016), 3 vom: 11. Feb., Seite 1106-19  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:209  |g year:2016  |g number:3  |g day:11  |g month:02  |g pages:1106-19 
856 4 0 |u http://dx.doi.org/10.1111/nph.13699  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 209  |j 2016  |e 3  |b 11  |c 02  |h 1106-19