Identification and regulation of host genes related to Rice stripe virus symptom production

Identification and regulation of host genes related to Rice stripe virus symptom production

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 209(2016), 3 vom: 11. Feb., Seite 1106-19
1. Verfasser: Shi, Bingbin (VerfasserIn)
Weitere Verfasser: Lin, Lin, Wang, Shihui, Guo, Qin, Zhou, Hong, Rong, Lingling, Li, Junmin, Peng, Jiejun, Lu, Yuwen, Zheng, Hongying, Yang, Yong, Chen, Zhuo, Zhao, Jinping, Jiang, Tong, Song, Baoan, Chen, Jianping, Yan, Fei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Nicotiana benthamiana Oryza sativa Rice stripe virus (RSV) chloroplast-related genes chlorosis eIF4A symptom development virus-derived siRNA mehr... Plant Proteins RNA, Messenger RNA, Small Interfering
Beschreibung
Zusammenfassung:© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
Viral infections cause plant chlorosis, stunting, necrosis or other symptoms. The down-regulation of chloroplast-related genes (ChRGs) is assumed to be responsible for chlorosis. We identified the differentially expressed genes (DEGs) in Rice stripe virus (RSV)-infected Nicotiana benthamiana, and examined the contribution of 75 down-regulated DEGs to RSV symptoms by silencing them one by one using Tobacco rattle virus (TRV)-induced gene silencing. Silencing of 11 of the 75 down-regulated DEGs caused plant chlorosis, and nine of the 11 were ChRGs. Silencing of a down-regulated DEG encoding the eukaryotic translation initiation factor 4A (eIF4A) caused leaf-twisting and stunting that were visible on RSV-infected N. benthamiana. A region of RSV RNA4 was complementary to part of eIF4A mRNA and virus-derived small interfering (vsiRNAs) from that region were present in infected N. benthamiana. When expressed as artificial microRNAs, those vsiRNAs could target NbeIF4A mRNA for regulation. We provide experimental evidence supporting the association of ChRGs with chlorosis and show that eIF4A is involved in RSV symptom development. This is also the first report demonstrating that siRNA derived directly from a plant virus can target a host gene for regulation
Beschreibung:Date Completed 13.12.2016
Date Revised 13.12.2023
published: Print-Electronic
GENBANK: AB026056.1, KP979608
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.13699