Changes in the starch-protein interface depending on common wheat grain hardness revealed using atomic force microscopy
Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 239(2015) vom: 20. Okt., Seite 1-8 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article Endosperm Microstructure Nanomechanics Puroindoline T. aestivum Plant Proteins Starch 9005-25-8 |
| Zusammenfassung: | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. The atomic force microscope tip was used to progressively abrade the surface of non-cut starch granules embedded in the endosperm protein matrix in grain sections from wheat near-isogenic lines differing in the puroindoline b gene and thus, hardness. In the hard near-isogenic wheat lines, starch granules exhibited two distinct profiles corresponding either to abrasion in the surrounding protein layer or the starch granule. An additional profile, only identified in soft lines, revealed a marked stop in the abrasion at the protein-starch transition similar to a lipid interface playing a lubricant role. It was related to the presence of both wild-type puroindolines, already suggested to act at the starch-protein interface through their association with polar lipids. This study revealed, for the first time, in situ differences in the nano-mechanical properties at the starch-protein interface in the endosperm of wheat grains depending on the puroindoline allelic status |
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| Beschreibung: | Date Completed 12.07.2016 Date Revised 10.03.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2015.07.006 |