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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b02026
|2 doi
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|a pubmed25n0840.xml
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|a (NLM)26305398
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Robertson, Neil M
|e verfasserin
|4 aut
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|a Discriminating a Single Nucleotide Difference for Enhanced miRNA Detection Using Tunable Graphene and Oligonucleotide Nanodevices
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|c 2015
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|a Text
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 08.07.2016
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|a Date Revised 27.11.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this study we have reported our efforts to address some of the challenges in the detection of miRNAs using water-soluble graphene oxide and DNA nanoassemblies. Purposefully inserting mismatches at specific positions in our DNA (probe) strands shows increasing specificity against our target miRNA, miR-10b, over miR-10a which varies by only a single nucleotide. This increased specificity came at a loss of signal intensity within the system, but we demonstrated that this could be addressed with the use of DNase I, an endonuclease capable of cleaving the DNA strands of the RNA/DNA heteroduplex and recycling the RNA target to hybridize to another probe strand. As we previously demonstrated, this enzymatic signal also comes with an inherent activity of the enzyme on the surface-adsorbed probe strands. To remove this activity of DNase I and the steady nonspecific increase in the fluorescence signal without compromising the recovered signal, we attached a thermoresponsive PEGMA polymer (poly(ethylene glycol) methyl ether methacrylate) to nGO. This smart polymer is able to shield the probes adsorbed on the nGO surface from the DNase I activity and is capable of tuning the detection capacity of the nGO nanoassembly with a thermoswitch at 39 °C. By utilizing probes with multiple mismatches, DNase I cleavage of the DNA probe strands, and the attachment of PEGMA polymers to graphene oxide to block undesired DNase I activity, we were able to detect miR-10b from liquid biopsy mimics and breast cancer cell lines. Overall we have reported our efforts to improve the specificity, increase the sensitivity, and eliminate the undesired enzymatic activity of DNase I on surface-adsorbed probes for miR-10b detection using water-soluble graphene nanodevices. Even though we have demonstrated only the discrimination of miR-10b from miR-10a, our approach can be extended to other short RNA molecules which differ by a single nucleotide
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|a Journal Article
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|a MicroRNAs
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|a Oligonucleotides
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|a Graphite
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|a 7782-42-5
|2 NLM
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|a Hizir, Mustafa Salih
|e verfasserin
|4 aut
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|a Balcioglu, Mustafa
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Rui
|e verfasserin
|4 aut
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|a Yavuz, Mustafa Selman
|e verfasserin
|4 aut
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|a Yumak, Hasan
|e verfasserin
|4 aut
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|a Ozturk, Birol
|e verfasserin
|4 aut
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|a Sheng, Jia
|e verfasserin
|4 aut
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|a Yigit, Mehmet V
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 31(2015), 36 vom: 15. Sept., Seite 9943-52
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:31
|g year:2015
|g number:36
|g day:15
|g month:09
|g pages:9943-52
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|u http://dx.doi.org/10.1021/acs.langmuir.5b02026
|3 Volltext
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