Dog erythrocyte antigen 1 : mode of inheritance and initial characterization
© 2015 American Society for Veterinary Clinical Pathology.
| Publié dans: | Veterinary clinical pathology. - 1975. - 44(2015), 3 vom: 01. Sept., Seite 369-79 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2015
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| Accès à la collection: | Veterinary clinical pathology |
| Sujets: | Journal Article Research Support, N.I.H., Extramural Blood groups blood typing canine polymorphism transfusion Antibodies, Monoclonal Blood Group Antigens Isoantibodies |
| Résumé: | © 2015 American Society for Veterinary Clinical Pathology. BACKGROUND: The dog erythrocyte antigen (DEA) 1 blood group system remains poorly defined OBJECTIVES: The purpose of the study was to determine the DEA 1 mode of inheritance and to characterize the DEA 1 antigen and alloantibodies ANIMALS: Canine research colony families, clinic canine patients, and DEA 1.2+ blood bank dogs were studied METHODS: Canine blood was typed by flow cytometry and immunochromatographic strips using anti-DEA 1 monoclonal antibodies. Gel column experiments with polyclonal and immunoblotting with monoclonal anti-DEA 1 antibodies were performed to analyze select samples. Cross-reactivity of human typing reagents against canine RBC and one monoclonal anti-DEA 1 antibody against human RBC panels was assessed RESULTS: Typing of 12 families comprising 144 dogs indicated an autosomal dominant inheritance with ≥ 4 alleles: DEA 1- (0) and DEA 1+ weak (1+), intermediate (2+), and strong (3+ and 4+). Samples from 6 dogs previously typed as DEA 1.2+ were typed as DEA 1+ or DEA 1- using monoclonal antibodies. Human typing reagents produced varied reactions in tube agglutination experiments against DEA 1+ and DEA 1- RBC. Polypeptide bands were not detected on immunoblots using a monoclonal anti-DEA 1 antibody, therefore the anti-DEA 1 antibody may be specific for conformational epitopes lost during processing CONCLUSIONS: The autosomal dominant inheritance of DEA 1 with ≥ 4 alleles indicates a complex blood group system; the antigenicity of each DEA 1+ type will need to be determined. The biochemical nature of the DEA 1 antigen(s) appears different from human blood group systems tested |
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| Description: | Date Completed 13.12.2016 Date Revised 13.11.2018 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/vcp.12284 |