Rapid method for the isolation of mammalian sperm DNA
The unique DNA packaging of spermatozoa renders them resistant to DNA isolation techniques used for somatic cells, requiring alternative methods that are slow and labor intensive. Here we present a rapid method for isolating high-quality sperm DNA. Isolated human sperm cells were homogenized with 0....
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 58(2015), 6 vom: 13. Juni, Seite 293-300
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| 1. Verfasser: |
Wu, Haotian
(VerfasserIn) |
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| Weitere Verfasser: |
de Gannes, Matthew K,
Luchetti, Gianna,
Pilsner, J Richard |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2015
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
DNA isolation
DNA purification
epigenetics
genetics
methods
nucleic acids
sperm
mehr...
spermatozoa
Phosphines
Reducing Agents
tris(2-carboxyethyl)phosphine
H49AAM893K
Silicon Dioxide
7631-86-9
DNA
9007-49-2 |
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