DOSY NMR and MALDI-TOF evidence of covalent binding the DNA duplex by trimethylammonium salts of topotecan upon near UV irradiation

Copyright © 2015 John Wiley & Sons, Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Magnetic resonance in chemistry : MRC. - 1985. - 53(2015), 8 vom: 27. Aug., Seite 565-71
1. Verfasser: Naumczuk, Beata (VerfasserIn)
Weitere Verfasser: Hyz, Karolina, Kawęcki, Robert, Bocian, Wojciech, Bednarek, Elżbieta, Sitkowski, Jerzy, Wielgus, Ewelina, Kozerski, Lech
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Magnetic resonance in chemistry : MRC
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA duplex MALDI-TOF MS NMR UV irradiation covalent binding topotecan Quaternary Ammonium Compounds Salts mehr... Topotecan 7M7YKX2N15 DNA 9007-49-2
Beschreibung
Zusammenfassung:Copyright © 2015 John Wiley & Sons, Ltd.
Using DOSY NMR and MALDI-TOF MS techniques, we present evidence that quaternary trimethylammonium salts of topotecan, [TPT-NMe3 ](+) X(-) (X = CF3SO3, HCOO), bind covalently the natural DNA oligomer upon near UV irradiation in water under physiological conditions. It is shown that formate salt is very reactive at pH 7 and requires short irradiation time. This weak irradiation at 365 nm paves the way for a new application of TPT derivatives in clinical use, which can dramatically increase the therapeutic effects of a medicine
Beschreibung:Date Completed 12.04.2016
Date Revised 23.07.2015
published: Print-Electronic
Citation Status MEDLINE
ISSN:1097-458X
DOI:10.1002/mrc.4255