Involvement of high mobility group box 1 in the activation of C5a-primed neutrophils induced by ANCA

Involvement of high mobility group box 1 in the activation of C5a-primed neutrophils induced by ANCA

Copyright © 2015 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 159(2015), 1 vom: 15. Juli, Seite 47-57
1. Verfasser: Wang, Chen (VerfasserIn)
Weitere Verfasser: Wang, Huan, Hao, Jian, Chang, Dong-Yuan, Zhao, Ming-Hui, Chen, Min
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ANCA C5a HMGB1 Neutrophils Antibodies, Antineutrophil Cytoplasmic HMGB1 Protein HMGB1 protein, human Complement C5a mehr... 80295-54-1 Peroxidase EC 1.11.1.7 Myeloblastin EC 3.4.21.76
Beschreibung
Zusammenfassung:Copyright © 2015 Elsevier Inc. All rights reserved.
C5a plays a central role in antineutrophil cytoplasmic antibody (ANCA)-mediated neutrophil recruitment and activation. A previous study showed that C5a played a crucial role in the regulation of high mobility group box 1 (HMGB1) release from human neutrophils. The current study further investigated the interaction between C5a and HMGB1 in ANCA-induced neutrophil activation. The effects of HMGB1 inhibitors on the translocation of ANCA antigens, ANCA-induced respiratory burst and degranulation of C5a-primed neutrophils were tested. We found that blocking HMGB1 decreased C5a-mediated translocation of ANCA antigens, as well as ANCA-induced respiratory burst and degranulation of C5a-primed neutrophils. Further study showed that supernatant of C5a-primed neutrophils, which contained HMGB1, also caused translocation of ANCA antigens of primary neutrophils, whereas blocking HMGB1 decreased the translocation. In conclusion, blocking HMGB1 may attenuate ANCA-induced activation of C5a-primed neutrophils. The interaction between HMGB1 and C5a might play an important role in ANCA-induced neutrophil activation
Beschreibung:Date Completed 24.08.2015
Date Revised 02.12.2018
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2015.04.008