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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1270/jsbbs.64.371
|2 doi
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Hoshino, Tomoki
|e verfasserin
|4 aut
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| 245 |
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|a A novel GmFAD3-2a mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.04.2015
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|a Date Revised 01.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Soybean (Glycine max (L.) Merr.) oil typically contains 8% α-linolenic acid that is highly unstable and easily oxidized. This property is undesirable in many food and industrial applications. Genetic strategies for reducing α-linolenic acid content would enhance the commercial value. However, genetic resources for low α-linolenic acid content are limited among natural soybean variations. Microsomal omega-3-fatty acid desaturase (FAD3) is responsible for the synthesis of α-linolenic acid in the polyunsaturated fatty acid pathway. There are four FAD3 homologs (Glyma02g39230, Glyma11g27190, Glyma14g37350 and Glyma18g06950) in the soybean genome. While non-functional alleles have been reported for Glyma02g39230 (GmFAD3-1a) and Glyma14g37350 (GmFAD3-1b), little variation is seen in Glyma18g06950 (GmFAD3-2a). We isolated seven mutant GmFAD3-2a alleles, each containing a single-nucleotide substitution, from 39,100 independent mutant lines by using targeting induced local lesions in genomes (TILLING). Analysis of GmFAD3-2a transcripts and enzyme activities revealed that one missense mutant, 'P1-A9', contains a non-functional allele of GmFAD3-2a. By combining three non-functional alleles (GmFAD3-1a, GmFAD3-1b, and GmFAD3-2a), we generated soybean lines containing <2% α-linolenic acid in their seeds. The reverse-genetics-based development of novel mutant alleles in the fatty acid metabolic pathway will allow the improvement of soybean with better oil quality through conventional breeding
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|a Journal Article
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| 650 |
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|a TILLING
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|a reverse genetics
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| 650 |
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|a soybean oil quality
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| 650 |
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4 |
|a α-linolenic acid
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| 700 |
1 |
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|a Watanabe, Satoshi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Takagi, Yutaka
|e verfasserin
|4 aut
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| 700 |
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|a Anai, Toyoaki
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Breeding science
|d 1998
|g 64(2014), 4 vom: 01. Dez., Seite 371-7
|w (DE-627)NLM098238299
|x 1344-7610
|7 nnns
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| 773 |
1 |
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|g volume:64
|g year:2014
|g number:4
|g day:01
|g month:12
|g pages:371-7
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|u http://dx.doi.org/10.1270/jsbbs.64.371
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