Association between cationic liposomes and low molecular weight hyaluronic acid

Association between cationic liposomes and low molecular weight hyaluronic acid

This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/H...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 31(2015), 11 vom: 24. März, Seite 3308-17
1. Verfasser: Gasperini, Antonio A M (VerfasserIn)
Weitere Verfasser: Puentes-Martinez, Ximena E, Balbino, Tiago Albertini, Rigoletto, Thais de Paula, Corrêa, Gabriela de Sá Cavalcanti, Cassago, Alexandre, Portugal, Rodrigo Villares, de La Torre, Lucimara Gaziola, Cavalcanti, Leide P
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Cations Liposomes Phosphatidylethanolamines 1,2-dielaidoylphosphatidylethanolamine 76391-83-8 Hyaluronic Acid 9004-61-9
Beschreibung
Zusammenfassung:This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential
Beschreibung:Date Completed 21.12.2015
Date Revised 24.03.2015
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la5045865