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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1111/nph.13064
|2 doi
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|a pubmed24n0807.xml
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Thor, Kathrin
|e verfasserin
|4 aut
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|a Cytosolic calcium signals elicited by the pathogen-associated molecular pattern flg22 in stomatal guard cells are of an oscillatory nature
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.05.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a CommentIn: New Phytol. 2014 Dec;204(4):733-5. - PMID 25367606
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|a Citation Status MEDLINE
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|a © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.
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|a Changes in cytosolic free calcium ([Ca(2+)]cyt) are an early and essential element of signalling networks activated by the perception of pathogen-associated molecular patterns (PAMPs), such as flg22. The flg22-induced calcium signal has been described on whole-plant, but not on single-cell scale so far. Also, the Ca(2+) sources and channels contributing to its generation are still obscure. Ratiometric fluorescence imaging employing the calcium reporter Yellow Cameleon 3.6 was performed to analyse the flg22-induced calcium signature in single guard cells of Arabidopsis thaliana. Calcium stores and channel types involved in its generation were determined by a pharmacological approach. In contrast to the calcium signal determined on whole-plant level, the signature on single-cell level is not characterized by one sustained response, but by oscillations in [Ca(2+)]cyt. These oscillations were abolished by EGTA and lanthanum, as well as by U73122, neomycin and TMB-8, but only partially or not at all affected by inhibitors of glutamate receptor-like channels and cyclic nucleotide-gated channels. Our analyses suggest that the response observed on whole-plant level is the summary of oscillations occurring in single cells. Parallel to external calcium, influx via channels located at internal stores contributes to the signal
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a Yellow Cameleon
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|a calcium
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|a calcium signal
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|a flagellin
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|a guard cells
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|a pathogen-associated molecular pattern (PAMP)
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|a plant-pathogen interactions
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|a Bacterial Proteins
|2 NLM
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|a Calcium-Binding Proteins
|2 NLM
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|a Enzyme Inhibitors
|2 NLM
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|a Estrenes
|2 NLM
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|a Fluorescent Dyes
|2 NLM
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|a Pyrrolidinones
|2 NLM
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|a yellow cameleon
|2 NLM
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|a 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
|2 NLM
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|a 112648-68-7
|2 NLM
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|a 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate
|2 NLM
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|a 57818-92-5
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|a Gallic Acid
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|a 632XD903SP
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|a Type C Phospholipases
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|a EC 3.1.4.-
|2 NLM
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|a Calcium
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|a Peiter, Edgar
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 204(2014), 4 vom: 01. Dez., Seite 873-81
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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|g volume:204
|g year:2014
|g number:4
|g day:01
|g month:12
|g pages:873-81
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|u http://dx.doi.org/10.1111/nph.13064
|3 Volltext
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