Cytosolic calcium signals elicited by the pathogen-associated molecular pattern flg22 in stomatal guard cells are of an oscillatory nature
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1979. - 204(2014), 4 vom: 01. Dez., Seite 873-81 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2014
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana Yellow Cameleon calcium calcium signal flagellin guard cells pathogen-associated molecular pattern (PAMP) plant-pathogen interactions mehr... |
| Zusammenfassung: | © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust. Changes in cytosolic free calcium ([Ca(2+)]cyt) are an early and essential element of signalling networks activated by the perception of pathogen-associated molecular patterns (PAMPs), such as flg22. The flg22-induced calcium signal has been described on whole-plant, but not on single-cell scale so far. Also, the Ca(2+) sources and channels contributing to its generation are still obscure. Ratiometric fluorescence imaging employing the calcium reporter Yellow Cameleon 3.6 was performed to analyse the flg22-induced calcium signature in single guard cells of Arabidopsis thaliana. Calcium stores and channel types involved in its generation were determined by a pharmacological approach. In contrast to the calcium signal determined on whole-plant level, the signature on single-cell level is not characterized by one sustained response, but by oscillations in [Ca(2+)]cyt. These oscillations were abolished by EGTA and lanthanum, as well as by U73122, neomycin and TMB-8, but only partially or not at all affected by inhibitors of glutamate receptor-like channels and cyclic nucleotide-gated channels. Our analyses suggest that the response observed on whole-plant level is the summary of oscillations occurring in single cells. Parallel to external calcium, influx via channels located at internal stores contributes to the signal |
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| Beschreibung: | Date Completed 15.05.2016 Date Revised 30.09.2020 published: Print-Electronic CommentIn: New Phytol. 2014 Dec;204(4):733-5. - PMID 25367606 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.13064 |