Insights from the cold transcriptome of Physcomitrella patens : global specialization pattern of conserved transcriptional regulators and identification of orphan genes involved in cold acclimation

© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 205(2015), 2 vom: 28. Jan., Seite 869-81
1. Verfasser: Beike, Anna K (VerfasserIn)
Weitere Verfasser: Lang, Daniel, Zimmer, Andreas D, Wüst, Florian, Trautmann, Danika, Wiedemann, Gertrud, Beyer, Peter, Decker, Eva L, Reski, Ralf
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Physcomitrella patens abiotic stress abscisic acid (ABA) cold acclimation microarray moss orphan genes transcriptome mehr... Abscisic Acid 72S9A8J5GW
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520 |a The whole-genome transcriptomic cold stress response of the moss Physcomitrella patens was analyzed and correlated with phenotypic and metabolic changes. Based on time-series microarray experiments and quantitative real-time polymerase chain reaction, we characterized the transcriptomic changes related to early stress signaling and the initiation of cold acclimation. Transcription-associated protein (TAP)-encoding genes of P. patens and Arabidopsis thaliana were classified using generalized linear models. Physiological responses were monitored with pulse-amplitude-modulated fluorometry, high-performance liquid chromatography and targeted high-performance mass spectrometry. The transcript levels of 3220 genes were significantly affected by cold. Comparative classification revealed a global specialization of TAP families, a transcript accumulation of transcriptional regulators of the stimulus/stress response and a transcript decline of developmental regulators. Although transcripts of the intermediate to later response are from evolutionarily conserved genes, the early response is dominated by species-specific genes. These orphan genes may encode as yet unknown acclimation processes 
650 4 |a Journal Article 
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650 4 |a abiotic stress 
650 4 |a abscisic acid (ABA) 
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700 1 |a Lang, Daniel  |e verfasserin  |4 aut 
700 1 |a Zimmer, Andreas D  |e verfasserin  |4 aut 
700 1 |a Wüst, Florian  |e verfasserin  |4 aut 
700 1 |a Trautmann, Danika  |e verfasserin  |4 aut 
700 1 |a Wiedemann, Gertrud  |e verfasserin  |4 aut 
700 1 |a Beyer, Peter  |e verfasserin  |4 aut 
700 1 |a Decker, Eva L  |e verfasserin  |4 aut 
700 1 |a Reski, Ralf  |e verfasserin  |4 aut 
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