Insights from the cold transcriptome of Physcomitrella patens : global specialization pattern of conserved transcriptional regulators and identification of orphan genes involved in cold acclimation
© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.
| Publié dans: | The New phytologist. - 1979. - 205(2015), 2 vom: 28. Jan., Seite 869-81 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2015
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| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Physcomitrella patens abiotic stress abscisic acid (ABA) cold acclimation microarray moss orphan genes transcriptome plus... |
| Résumé: | © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust. The whole-genome transcriptomic cold stress response of the moss Physcomitrella patens was analyzed and correlated with phenotypic and metabolic changes. Based on time-series microarray experiments and quantitative real-time polymerase chain reaction, we characterized the transcriptomic changes related to early stress signaling and the initiation of cold acclimation. Transcription-associated protein (TAP)-encoding genes of P. patens and Arabidopsis thaliana were classified using generalized linear models. Physiological responses were monitored with pulse-amplitude-modulated fluorometry, high-performance liquid chromatography and targeted high-performance mass spectrometry. The transcript levels of 3220 genes were significantly affected by cold. Comparative classification revealed a global specialization of TAP families, a transcript accumulation of transcriptional regulators of the stimulus/stress response and a transcript decline of developmental regulators. Although transcripts of the intermediate to later response are from evolutionarily conserved genes, the early response is dominated by species-specific genes. These orphan genes may encode as yet unknown acclimation processes |
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| Description: | Date Completed 24.08.2015 Date Revised 21.03.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.13004 |