Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan
A method for conserving primers and differently labeled fluorogenic hydrolysis (i.e., TaqMan) probes at ambient conditions is presented. Primers and hydrolysis probes with four different fluorophore-quencher combinations (6- FAM-BHQ1, HEX-BHQ1, ROX-BHQ650, and Cy5-BHQ2) were mixed with trehalose and...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1991. - 57(2014), 3 vom: 11., Seite 151-5
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| Auteur principal: |
Rombach, Markus
(Auteur) |
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| Autres auteurs: |
Kosse, Dominique,
Faltin, Bernd,
Wadle, Simon,
Roth, Günter,
Zengerle, Roland,
von Stetten, Felix |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2014
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, Non-U.S. Gov't
air-drying
preservation
primer
probe
stability testing
trehalose
xanthan
DNA Primers
plus...
Excipients
Fluorescent Dyes
Polysaccharides, Bacterial
Trehalose
B8WCK70T7I
xanthan gum
TTV12P4NEE |
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