Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan
A method for conserving primers and differently labeled fluorogenic hydrolysis (i.e., TaqMan) probes at ambient conditions is presented. Primers and hydrolysis probes with four different fluorophore-quencher combinations (6- FAM-BHQ1, HEX-BHQ1, ROX-BHQ650, and Cy5-BHQ2) were mixed with trehalose and...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 57(2014), 3 vom: 11., Seite 151-5
|
|---|
| 1. Verfasser: |
Rombach, Markus
(VerfasserIn) |
|---|
| Weitere Verfasser: |
Kosse, Dominique,
Faltin, Bernd,
Wadle, Simon,
Roth, Günter,
Zengerle, Roland,
von Stetten, Felix |
|---|
| Format: | Online-Aufsatz
|
|---|
| Sprache: | English |
|---|
| Veröffentlicht: |
2014
|
|---|
| Zugriff auf das übergeordnete Werk: | BioTechniques
|
|---|
| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
air-drying
preservation
primer
probe
stability testing
trehalose
xanthan
DNA Primers
mehr...
Excipients
Fluorescent Dyes
Polysaccharides, Bacterial
Trehalose
B8WCK70T7I
xanthan gum
TTV12P4NEE |
|---|